IL-2 Monoclonal Antibody (MQ1-17H12), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
IL-2
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
aldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Symbols
IL2
Primary or Secondary
Primary
Content And Storage
4° C
Gene
IL2
Clone
MQ1-17H12
Applications
ELISA, Flow Cytometry
Conjugate
Unconjugated
Host Species
Rat
Target Species
Human
Gene Accession No.
P60568
Gene ID (Entrez)
3558
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine
- The MQ1-17H12 antibody is a non-neutralizing antibody
- Applications Reported: The MQ1-17H12 antibody has been reported for use as capture antibody for ELISA and for intracellular staining for flow cytometric analysis
- Fluorochrome conjugated MQ1-17H12 antibody is recommended for use in intracellular staining for flow cytometry
- Applications Tested: The MQ1-17H12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-2 (IL-2) in combination with the biotin anti-human IL-2 poly (13-7028) antibody for detection and recombinant human IL-2 (14-8029) as the standard
- A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL
- A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate
- Purity: Greater than 90%, as determined by SDS-PAGE
- Aggregation: Less than 10%, as determined by HPLC
- Filtration: 0.2 μm post-manufacturing filtered
- Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages
- IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7)
- The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene
- The targeted disruption of a gene similar to IL-2 in mice leads to an μgcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.