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7069789

IgM Monoclonal Antibody (II/41), PE-Cyanine5, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

IgM

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

AGM1; AI326478; Igh6; Igh-6; Ighm; Igh-M; Igm; immunoglobulin heavy constant mu; MU; muH; VH

Gene Symbols

Ighm

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Ighm

Clone

II/41

Applications

Flow Cytometry

Conjugate

PE-Cyanine5

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P01872

Gene ID (Entrez)

16019

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM
  • It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA
  • IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells
  • Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM
  • Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 0.125 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Light sensitivity: This tandem dye is sensitive photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
  • 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
  • Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen
  • It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum
  • Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens
  • It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated
  • Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.