CD11c Monoclonal Antibody (BU15), APC, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD11c
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
AI449405; CD11 antigen-like family member C; CD11c; CD11C (p150) alpha polypeptide; complement component 3 receptor 4 subunit; complement receptor 4; Cr4; integrin alpha X; integrin alpha-X; integrin aX; integrin subunit alpha X; integrin, alpha X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); Itgax; Leu M5; leu M5, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; N418; p150 95 integrin alpha chain; RGD1561123; SLEB6
Gene Symbols
Itgax
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Itgax
Clone
BU15
Applications
Flow Cytometry
Conjugate
APC
Host Species
Mouse
Target Species
Canine, Cynomolgus Monkey, Human, Non-human Primate
Gene Accession No.
P20702
Gene ID (Entrez)
102147272, 3687, 489929
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The BU15 monoclonal antibody reacts with canine, human, and non-human primate CD11c, the 150 kDa integrin aX chain
- CD11c non-covalently associates with β2 integrin to form the CD11c/CD18 heterodimer
- This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells, and subset of T and B lymphocytes
- CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions
- CD11c also binds complement fragment iC3b to mediate phagocytosis of opsonized particles
- The BU15 antibody has been reported to crossreact to cynomologus monkey
- Moreover, this antibody has been reported to exhibit blocking activity
- Crossblocking studies suggest that BU15 recognizes a different epitope from clone 3.9
- Applications Reported: This BU15 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This BU15 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
- This can be used at 5 μL (0.06 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
- Filtration: 0.2 μm post-manufacturing filtered
- CD11, along with CD18, form a heterodimer adhesion molecule
- In particular, CD11 is composed of CD11a, CD11b and CD11c
- CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils
- CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes
- CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.