CD135 (Flt3) Monoclonal Antibody (A2F10), APC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD135 (Flt3)
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
B230315G04; CD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK2; Flk-2; FLT3; Flt-3; fms related tyrosine kinase 3; FMS-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Ly72; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; stem cell tyrosine kinase 1; STK1; STK-1; tyrosine-protein kinase FLT3; tyrosine-protein kinase receptor flk-2; wmfl
Gene Symbols
FLT3
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
FLT3
Clone
A2F10
Applications
Flow Cytometry
Conjugate
APC
Host Species
Rat
Target Species
Mouse
Gene Accession No.
Q00342
Gene ID (Entrez)
14255
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
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Description
- Description: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3
- A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow
- Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively
- Applications Reported: This A2F10 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
- This can be used at less than or equal to 1 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
- Filtration: 0.2 μm post-manufacturing filtered
- FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family
- The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor
- The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow
- Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.