TCR V beta 5.1/5.2 Monoclonal Antibody (MR9-4), APC, eBioscience™, Invitrogen™

Description

• Description: This MR9-4 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 5.1/5.2 chain
• Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement
• The MR9-4 antibody recognizes the V beta 5.1 and 5.2 chains on T cells from mouse strains with the b haplotype of the Tcrb gene, including C57BL/6, B10, and C58/J
• V beta 5.1 and 5.2 are deleted in mouse strains that express MHC Class II I-E, including Balb/c, C3H, and DBA/2
• Reports also indicate that V beta 5+ T cells recognize the OVA peptide
• Applications Reported: This MR9-4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MR9-4 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• TCR V beta 5.1/5.2 Monoclonal antibody specifically detects TCR V beta 5.1/5.2 in Mouse samples
• It is validated for Flow Cytometry.