Home Products Antibodies Primary Antibodies 7090548
7090548

CD4 Monoclonal Antibody (RM4-5), eFluor™ 570, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD4

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen

Gene Symbols

CD4

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

CD4

Clone

RM4-5

Applications

Immunocytochemistry, Immunohistochemistry (Frozen)

Conjugate

eFluor 570

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P06332

Gene ID (Entrez)

12504

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells
  • CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells
  • In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail
  • Binding of RM4-5 is blocked by GK1.5
  • Applications Reported: This RM4-5 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, microscopy, and immunocytochemistry
  • Applications Tested: This RM4-5 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 5 μg/mL
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70)
  • A standard Alexa Fluor™ 555 or TRITC filter is acceptable
  • Excitation: 555 nm; Emission: 570 nm The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV
  • CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells
  • At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues
  • CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck
  • It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system
  • Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].