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7090589

CD15 Monoclonal Antibody (HI98), eFluor™ 615, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD15

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

FUTIV, Lewis X, SSEA-1

Gene Symbols

FUT4

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

FUT4

Clone

HI98

Applications

Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Conjugate

eFluor 615

Host Species

Mouse

Target Species

Human

Gene Accession No.

P22083

Gene ID (Entrez)

2526

Isotype

IgM κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X
  • This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes
  • Monocytes express this structure at varying degree while lymphocytes are negative
  • Applications Reported: This HI98 antibody has been reported for use in immunohistochemical staing of frozen and FFPE tissue sections (IHC-F, IHC-P), and immunocytochemical staining
  • Applications Tested: This HI98 antibody has been tested by immunohistochemical staining of formalin-fixed paraffin embedded human human kidney cortex (IHC-P) using high pH antigen retrieval at less than or equal to 5 μg/mL
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • This product has not been validated for flow cytometric analysis
  • Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75)
  • A standard Alexa Fluor™ 594 filter is acceptable
  • Excitation: 595 nm; Emission: 615 nm
  • Filtration: 0.2 μm post-manufacturing filtered
  • CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g
  • granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells
  • There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x
  • Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction
  • This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells
  • Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins
  • CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.