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7090661

IL-10 Monoclonal Antibody (JES5-16E3), PerCP-Cyanine5.5, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

IL-10

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF

Gene Symbols

IL10

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

IL10

Clone

JES5-16E3

Applications

Flow Cytometry

Conjugate

PerCP-Cyanine5.5

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P18893

Gene ID (Entrez)

16153

Isotype

IgG2b κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10)
  • Mouse IL-10 is an ∽18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF)
  • In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10
  • IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response
  • Applications Reported: This JES5-16E3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
  • Applications Tested: This JES5-16E3 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes
  • This can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes
  • This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages
  • It also enhances B cell survival, proliferation, and antibody production
  • Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway
  • Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract
  • Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis
  • Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.