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7090662

IL-12/IL-23 p40 Monoclonal Antibody (C17.8), PerCP-Cyanine5.5, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

IL-12/IL-23 p40

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

CLMF; CLMF p35; CLMF p40; CLMF2; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; Il12; Il-12 p35; IL12 p40; IL23; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL12, subunit p40; Il12a; IL-12A; Il12b; IL-12B; IL12p35; IL-12p35; Il12p40; Il-12p40; il23; IL-23; IL23 p41; IL-23 subunit alpha; Il23a; IL-23A; IL-23-A; IL23P19; IL-23p19; IL35 subunit; ILN; IMD28; IMD29; Interleukin; Interleukin 12; interleukin 12 35 kDa subunit; interleukin 12 35kDa subunit; interleukin 12 40 kDa subunit; interleukin 12 40kDa subunit; interleukin 12 p35 subunit; interleukin 12 polypeptide 35; interleukin 12 subunit p40; interleukin 12, p35; interleukin 12, p40; interleukin 12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12b; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23 p19 subunit; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; Interleukin12; interleukin-12 alpha chain; interleukin-12 beta chain; interleukin-12 p40; interleukin-12 p40 subunit; interleukin-12 subunit alpha; Interleukin-12 subunit beta; interleukin-12 subunit p40; interleukin-23 p19 subunit; interleukin-23 p40 subunit; interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; Ll12a; natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; p19; P35; p40; RP23-388G23.1; SGRF; UNQ2498/PRO5798

Gene Symbols

IL12A, IL12B, Il23a

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Clone

C17.8

Applications

Flow Cytometry

Conjugate

PerCP-Cyanine5.5

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P43431, P43432, Q9EQ14

Gene ID (Entrez)

16159, 16160, 83430

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23
  • The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity
  • For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19)
  • IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells
  • IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells
  • Applications Reported: This C17.8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
  • Applications Tested: This C17.8 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes
  • This can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation
  • IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40)
  • IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit
  • Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue
  • In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.