Home Products Antibodies Primary Antibodies 7090780
7090780

CD170 (Siglec F) Monoclonal Antibody (1RNM44N), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD170 (Siglec F)

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

CD170; CD33 antigen-like 2; CD33L2; mSiglec-F; OB-binding protein 2; OBBP2; OB-BP2; Obesity-binding protein 2; sialic acid binding Ig like lectin 5; sialic acid binding Ig-like lectin 5; sialic acid binding Ig-like lectin F; sialic acid-binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin F; sialic acid-binding immunoglobulin-like lectin 5; sialic acid-binding lectin Siglec-F; Siglec; SIGLEC5; siglec-5; Siglecf; Siglec-F

Gene Symbols

Siglecf

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Siglecf

Clone

1RNM44N

Applications

Flow Cytometry

Conjugate

PerCP-eFluor 710

Host Species

Rat

Target Species

Mouse

Gene Accession No.

Q920G3

Gene ID (Entrez)

233186

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F
  • Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types
  • The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding
  • Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells
  • Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells
  • This 1RNM44N antibody will recognize a formaldehyde-fixed epitope
  • Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells
  • This can be used at less than or equal to 0.125 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells
  • This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid
  • The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface
  • This molecule is expressed by monocytic/myeloid lineage cells
  • SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus
  • It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.