IgD Monoclonal Antibody (11-26c (11-26)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD
• It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM
• IgD is expressed by peripheral mature B cells
• 11-26c does not activate B cells
• Applications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Please make sure that your instrument is capable of detecting this fluorochrome
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM
• IgD also exists as a soluble form in blood serum
• Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.