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7090923

KLRG1 Monoclonal Antibody (13F12F2), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

KLRG1

Concentration

5 μL/Test

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias

2F1; 2F1-Ag; CLEC15A; C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; killer cell lectin like receptor G1; killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; Klrg1; Mafa; MAFA-2F1; MAFAL; MAFA-L; MAFA-LIKE; MAFA-like receptor; Mast cell function-associated antigen; mast cell function-associated antigen (ITIM-containing); mast cell function-associated antigen 2F1; natural killer cell receptor

Gene Symbols

Klrg1

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Klrg1

Clone

13F12F2

Applications

Flow Cytometry

Conjugate

PerCP-eFluor 710

Host Species

Mouse

Target Species

Human

Gene Accession No.

Q96E93

Gene ID (Entrez)

10219

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitor receptor of the C-type lectin superfamily that contains an ITIM domain
  • This inhibitory receptor is expressed on subsets of gamma-delta T cells, NK (CD56dim), CD8+ and CD4+ T Cells
  • KLRG1 is expressed primarily by cells with an effector/memory phenotype that are short-lived, but capable of immediate effector cell function
  • Cadherin-E, -N, and -R are ligands for KLRG1
  • Cadherin/KLRG1 interaction inhibits cytolytic activity and proliferation
  • The percentage of KLRG1 positive cells can vary considerably, depending on antigen experience
  • The clones 13F12F2 and 13A2 appear to recognize a similar epitope based on cross-blocking studies
  • Applications Reported: This 13F12F2 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 13F12F2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
  • This can be used at 5 μL (0.25 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
  • KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains
  • These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1
  • KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells
  • NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.