CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule
• CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains
• These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others
• Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1
• CD90 is involved in regulation of adhesion and signal transduction by T cells
• Applications Reported: This 53-2.1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.015 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family
• CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts
• Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain
• CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines
• In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.