AIRE Monoclonal Antibody (5H12), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE)
• This 58-kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs)
• However, expression of AIRE protein in the periphery remains unclear despite the detection of AIRE mRNA in the lymph node, lymphoid stromal cells, monocytes, and dendritic cells
• Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection
• Other biological functions for AIRE include chemokine expression and antigen presentation
• The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2
• Applications Reported: This 5H12 antibody has been reported for use in intracellular flow cytometric analysis and immunohistochemical staining of frozen tissue sections
• Applications Tested: This 5H12 antibody has been tested by immunohistochemistry on frozen 5-week-old mouse thymus and can be used at less than or equal to 10 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• AIRE is a transcriptional regulator protein that binds to DNA as a dimer and tetramer, but not as a monomer, and may be involved in the regulation of gene expression
• A nuclear protein, AIRE is a 545 amino acid protein with an HSR domain, two PHD type zinc fingers, and a SAND domain, and may be present in soluble high-molecular-weight complexes
• It may form homodimer, homotetramer and also nuclear bodies and interacts with the transcriptional coactivator CBP and CREBBP
• Three isoforms of AIRE are reported and one of them is predicted to be a candidate for nuclear-mediated decay (NMD)
• AIRE is involved in immune regulation and is known to mediate E3 ubiquitin ligase activity
• Defects in AIRE are responsible for the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).