Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor™ 700, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Ki-67
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05
Gene Symbols
Mki67
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Mki67
Clone
SolA15
Applications
Flow Cytometry
Conjugate
Alexa Fluor 700
Host Species
Rat
Target Species
Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat
Gene Accession No.
E9PVX6, P46013
Gene ID (Entrez)
100686578, 102135895, 17345, 291234, 4288
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein
- Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0)
- Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis
- Ki-67 is used as a marker for determining the growth fraction of a given population of cells
- In studies of tumor cells, the Ki-67 labeling index refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types
- Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin)
- The SolA15 antibody also recognizes human, non-human primate and canine Ki-67
- Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This SolA15 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat
- 00-5523) and protocol
- Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online
- This antibody can be used at less than or equal to 0.25 μg per test
- Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases
- The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus
- Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase
- Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis
- Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms
- In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index
- The correlation between low Ki-67 index and histologically low-grade tumors is strong
- Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.