Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), PE, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Phospho-AKT1 (Ser473)
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
Akt; Akt kinase; AKT serine/threonine kinase 1; Akt/PKB; akt1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; CAKT; C-AKT; CG4006; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; dAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAKT1; DAKT1/PKB; Dmel\CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; P-AKT; PKB; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; protein kinase B; Protein kinase B alpha; protein kinase B-alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine protein kinase; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; SD10374p; serine/threonine protein kinase; serine-threonine protein kinase; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Gene Symbols
AKT1
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
AKT1
Clone
SDRNR
Applications
Flow Cytometry
Conjugate
PE
Host Species
Mouse
Target Species
Human, Mouse
Gene Accession No.
P31749, P31750
Gene ID (Entrez)
11651, 207
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
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Description
- Description: This SDRNR monoclonal antibody recognizes human and mouse AKT (also known as Protein Kinase B (PKB)) when phosphorylated on S473
- AKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration
- There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3
- AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation
- Phosphorylation of AKT at T308 by PDK1 and at S473 is required for full activation of this kinase
- AKT promotes cell survival by inhibiting apoptosis via phosphorylation and inactivation of several targets including Bad, Foxo1, c-Raf, and caspase-9
- Deregulation of AKT has been implicated as a major contributing factor in many types of cancer
- AKT is negatively regulated by the phosphatase PTEN as well as by the chemical inhibitor LY294002
- Specificity of this SDRNR clone was determined by ELISA, flow cytometry, and western blotting
- Applications Reported: This SDRNR antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This SDRNR antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells
- This can be used at 5 μL (0.06 μg) per test
- AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival
- The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells
- This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors
- Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9
- The AKT pathway is a major target for cancer drug discovery.