CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1
• B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily
• B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC
• B7-H1 is a ligand for PD-1
• Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses
• MIH5 is reported to be a blocking antibody
• Applications Reported: This MIH5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Please make sure that your instrument is capable of detecting this fluorochrome
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member
• To date, one specific receptor has been identified that can be ligated by PD-L1
• This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling
• Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production
• PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation
• Thus, PD-L1 might play an important role in tumor immune evasion.