7092484
CD23 Rat anti-Mouse, PE, Clone: B3B4, BD
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD23
Concentration
0.2mg/mL
Classification
Monoclonal
Host Species
Rat
Target Species
Mouse
Isotype
IgG2a κ
Purification Method
Affinity Purified
Description
FcεRII; Fc-epsilon-RII; Fcer2a; Ly-42; Low-affinity IgE receptor; Fcer2
Clone
B3B4
Applications
Flow Cytometry
Conjugate
PE
Regulatory Status
RUO
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Related Products
Description
- The B3B4 antibody reacts with CD23, the low affinity IgE Fc receptor (FcεRII) expressed on mature resting conventional B lymphocytes, but not on B-1 cells (CD5+ B cells) or T lymphocytes
- It does not react with high-affinity IgE receptors, as demonstrated on mouse mast cell lines
- The regulation of CD23 surface expression on activated B cells appears to be complex, depending upon the mode of activation and the presence of cytokines
- IgE synthesis is negatively regulated by CD23, and CD23 expression is upregulated on splenocytes in the presence of IgE
- CD23 is also upregulated on follicular dendritic cells in the lymph nodes of immunized mice, and a subset of splenic dendritic cells expresses CD23
- The B3B4 antibody abrogates antigen-specific IgE-dependent modulation of immune responses in normal mice
- This monoclonal antibody also blocks IgE binding and eosinophil infiltration in the lung of immunized mice
- Different in vivo results have been obtained when using the intact B3B4 antibody or the F(ab')2 fragments
- B3B4 mAb does not cross-react with rat or human IgE Fc Receptor.