7092776
Ly-6A/E Rat anti-Mouse, PE-Cy7, Clone: D7, BD
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Ly-6A/E
Concentration
0.2mg/mL
Classification
Monoclonal
Host Species
Rat
Target Species
Mouse
Immunogen
IL-2-dependent mouse T-cell line CTL-L
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Clone
D7
Applications
Flow Cytometry
Conjugate
PE-Cyanine7
Regulatory Status
RUO
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype
IgG2a κ
Purification Method
Affinity Purified
Description
Ly-6A/E; Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Sca-1; TAP
Related Products
Description
- The D7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family
- Sca-1 (Ly6A/E), a phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cells (HSC) in the bone marrow of mice with both Ly-6 haplotypes
- In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes and thymic and peripheral T lymphocytes
- Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of lymphocytes from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen
- Studies with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation
- Purified E13-161.7 mAb (anti-Ly-6A/E, Cat
- No
- 553333) can block binding of FITC-conjugated D7 antibody (Cat
- No
- 557405) to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated E13-161.7 antibody (Cat
- No
- 553335)
- Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel (Cat
- No
- 559971) to identify HSC.