TNF-alpha Mouse, Clone: TNF706, Novus Biologicals™

TNF-alpha

Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 1-2ug/ml, Immunohistochemistry-Frozen 1-2ug/ml

Monoclonal

Purified

RUO

Human, Mouse, Rat, Canine, Feline, Rabbit, Zebrafish

P01375

7124

Recombinant N-terminal fragment of human TNF-alpha

Primary

Store at 4C.

17 kDa

TNF706

Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Unconjugated

Mouse

Adaptive Immunity, Asthma, Autophagy, Biologically Active Proteins, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity

PBS with 0.05% BSA. with 0.05% Sodium Azide

APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

TNF

IgM κ

Protein A purified

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.