EMI1 Antibody (EMI1/1176) - Azide and BSA Free, Novus Biologicals™

EMI1

1.0 mg/mL

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Unconjugated

Mouse

Human

EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31

FBXO5

IgG2a κ

Protein A or G purified

It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

EMI1/1176

Western Blot : 1 - 2 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Monoclonal

Purified

RUO

PBS with No Preservative

26271

Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Primary

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

56 kDa