7105860

EpCAM/TROP1 Antibody (EGP40/1120), Biotin - (ECD), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

EpCAM/TROP1

Concentration

1.0 mg/mL

Applications

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cancer, Cancer Stem Cells

Formulation

PBS with No Preservative

Gene ID (Entrez)

4072

Immunogen

Recombinant full-length human EpCAM protein

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen

41 kDa

Clone

EGP40/1120

Dilution

Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse (Negative), Rat (Negative)

Gene Alias

17-1A, 323/A3, ACSTD1, antigen identified by monoclonal AUA1, CD326 antigen, Cell surface glycoprotein Trop-1, chromosome 4, surface marker (35kD glycoprotein), DIAR5, EGP, EGP-2, EGP314, EGP40, EpCAM, epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733-2EGP34, hEGP314, HNPCC8, KS 1/4 antigen, KS1/4, KSAHEA125, M1S2, M4S1Ly74, Major gastrointestinal tumor-associated protein GA733-2, MIC18MH99, MOC31, TACST-1, TACSTD1, TROP1CD326, Tumor-associated calcium signal transducer 1CO-17A

Gene Symbols

EPCAM

Isotype

IgG1 κ

Purification Method

Protein A or G purified

Test Specificity

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

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Description

  • EpCAM/TROP1 Monoclonal specifically detects EpCAM/TROP1 in Human, Mouse (Negative), Rat (Negative) samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.