Alkaline Phosphatase/ALPP Antibody (ALPP/516) - Azide and BSA Free, Novus Biologicals™

Alkaline Phosphatase/ALPP

1.0 mg/mL

Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence, CyTOF

Unconjugated

Mouse

Cancer, Embryonic Stem Cell Markers, Ovarian Carcinoma Cell Markers, Stem Cell Markers

PBS with No Preservative

250

Recombinant full-length human ALPP protein

Primary

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

70 kDa

ALPP/516

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen : 1.0 - 2.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Monoclonal

Purified

RUO

Human

Alkaline phosphatase Regan isozyme, alkaline phosphatase, placental, alkaline phosphatase, placental (Regan isozyme), alkaline phosphatase, placental type, alkaline phosphomonoesterase, ALP, EC 3.1.3.1, FLJ61142, glycerophosphatase, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1, Regan isozyme

ALPP

IgG1 κ

Protein A or G purified

PLAP is a tissue specific, trophoblast-derived, 70kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo-glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non-tissue specific AP is found in kidney, liver and bone. This MAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP and to some variants of AP from normal human testis. This MAb can be used as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes.