7106124

CD8 Antibody (4B11), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 4 publications

Manufacturer: Fischer Scientific

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Antigen

CD8

Dilution

Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:20-1:40, Immunohistochemistry-Frozen 1:20-1:40, Dual RNAscope ISH-IHC

Classification

Monoclonal

Form

Supernatant

Regulatory Status

RUO

Target Species

Human

Gene Accession No.

P01732

Gene ID (Entrez)

925

Immunogen

Synthetic peptide derived from the carboxy terminal region of the humanCD8 alpha chain coupled to a N-terminal cysteine, with the sequence C-KSDGKPSLSARYV. The peptide was coupled to bovine serum albuman and keyhole limpet hemocyanin.

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen

26 kDa

Clone

4B11

Applications

Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), In Situ Hybridization (ISH)

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction, Stem Cell Markers

Formulation

Tissue culture supernatant with 0.09% Sodium Azide

Gene Alias

CD8, CD8a molecule, LEU2, p32

Gene Symbols

CD8A

Isotype

IgG2b

Purification Method

Tissue culture supernatant

Test Specificity

Recognizes the human CD8 cell surface antigen, a 32kD glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. Clone 4B11 has been reported as being suitable for use in Western blotting.

Description

  • CD8 Monoclonal specifically detects CD8 in Human samples
  • It is validated for Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Dual RNAscope ISH-IHC.