CD166 (ALCAM) Monoclonal Antibody (3A6), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F
• Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types
• The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding
• Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells
• Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells
• This 1RNM44N antibody will recognize a formaldehyde-fixed epitope
• Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages
• This may be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain
• This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration
• Multiple alternatively spliced transcript variants encoding different isoforms have been found.