7116504

Human/Mouse/Rat Vimentin Antibody, R&D Systems™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Vimentin

Dilution

Western Blot 2 ug/mL, Immunohistochemistry 5-25 ug/mL, Immunocytochemistry 8-25 ug/mL, Knockout Validated 5-15 ug/mL, Dual RNAscope ISH-IHC 5-25 ug/mL

Classification

Monoclonal

Form

Supernatant

Target Species

Human, Mouse, Rat

Gene Accession No.

P08670

Gene ID (Entrez)

7431

Isotype

IgG2a

Purification Method

Protein A or G purified from hybridoma culture supernatant

Content And Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Clone

979517

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoassay, In Situ Hybridization (ISH)

Conjugate

Unconjugated

Host Species

Mouse

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

Gene Alias

FLJ36605, VIM, vimentin

Immunogen

E.coli-derived recombinant human Vimentin Ser2-Glu466 Accession # P08670

Primary or Secondary

Primary

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity

Detects human Vimentin in direct ELISAs. Detects human, mouse, and rat Vimentin in Western blots.

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Description

  • Vimentin Monoclonal specifically detects Vimentin in Human, Mouse, Rat samples
  • It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry, Knockout Validated, Dual RNAscope ISH-IHC.