Bcl-6 Mouse anti-Human, Clone: BCL6/1475, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 1 publication
Manufacturer: Fischer Scientific
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Antigen
Bcl-6
Concentration
0.2 mg/mL
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, SDS-Page
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cancer, Cell Cycle and Replication, Tumor Suppressors
Formulation
10mM PBS with 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez)
604
Immunogen
Recombinant human bcl-6 protein fragment (aa256-389)
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
95 kDa
Clone
BCL6/1475
Dilution
Western Blot 0.5-1 ug/ml, Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 1-2 ug/ml, SDS-Page, Protein Array 1:100-1:2000
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
B-cell CLL/lymphoma 6, B-cell lymphoma 6 protein transcript, BCL-5, BCL5B-cell lymphoma 5 protein, BCL-6, BCL6A, cys-his2 zinc finger transcription factor, LAZ3ZBTB27B-cell lymphoma 6 protein, lymphoma-associated zinc finger gene on chromosome 3, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51ZNF51, zinc finger transcription factor BCL6S
Gene Symbols
BCL6
Isotype
IgG1 κ
Purification Method
Protein A or G purified
Test Specificity
The specificity of this monoclonal antibody to its intended target was validated by HuProt(TM) Array, containing more than 19,000, full-length human proteins. Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma
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Description
- Bcl-6 Monoclonal specifically detects Bcl-6 in Human samples
- It is validated for Western Blot, Flow Cytometry, Protein Array.