CD48 Monoclonal Antibody (HM48-1), Super Bright™ 702, eBioscience™, Invitrogen™
Armenian Hamster Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD48
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias
AI449234; AW610730; B-cell activation marker; BCM1; Bcm-1; BCM1 surface antigen; BLAST; BLAST1; BLAST-1; B-lymphocyte activation marker BLAST-1; Cd48; CD48 antigen; CD48 antigen (B cell membrane protein); CD48 antigen (B-cell membrane protein); Cd48 molecule; CD48d; hCD48; HM48-1; leukocyte antigen MEM-102; mCD48; MEM-102; MRC OX-45 surface antigen; sCD 48; sCD48; Sgp-60; Signaling lymphocytic activation molecule 2; Signaling lymphocytic activation molecule 2 (SLAMF2); SLAM family member 2; SLAMF2; soluble CD 48; soluble CD48; TCT.1
Gene Symbols
Cd48
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Cd48
Clone
HM48-1
Applications
Flow Cytometry
Conjugate
Super Bright 702
Host Species
Armenian Hamster
Target Species
Mouse
Gene Accession No.
P18181
Gene ID (Entrez)
12506
Isotype
IgG
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit
- The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106)
- This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils
- CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1)
- Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis
- Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 1 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm
- We recommend using a 710/50 bandpass filter
- Please make sure that your instrument is capable of detecting this fluorochrome
- When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
- SB-4400) to minimize any non-specific polymer interactions
- CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily that includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others
- These molecules bind to the same or another members of the Ig family, thus mediating homotypic or heterotypic adhesion
- CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2
- 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation
- Further, signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases
- CD48 is strongly expressed on lymphocytes and monocytes, weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium
- CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2
- 2B4-CD48 interaction may be involved in the pathology of allergic airway diseases and provide a pharmacological target for the treatment of diseases such as allergic rhinitis and asthma.