TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), Super Bright™ 780, eBioscience™, Invitrogen™

Description

• Description: This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170-210 kDa member of the TAM family of tyrosine kinase receptors that also includes Axl and Tyro3
• MerTK is expressed on tissue macrophages and is involved in the removal of apoptotic cells
• This process relies on two soluble ligands of MerTK, Protein S and Gas6 that bind to phosphatidylserine found on the outer leaflet of the plasma membrane of cells undergoing apoptosis
• Upon binding these ligands, MerTK undergoes autophosphorylation at multiple tyrosine residues that activate the PI3K and Akt pathways
• This results in the phagocytosis of apoptotic cells and also results in the direct inhibition of TLR-induced production of pro-inflammatory cytokines
• In addition, MerTK may function as a putative entry receptor for filoviruses
• Deficiency of MerTK causes general autoimmunity, inflammation and accumulation of apoptotic bodies
• MerTK is constitutively released from the cell surface by metalloproteinases and thus is present in the serum and culture medium
• This process can be enhanced by stimulation with LPS
• MerTK can be used to help discriminate macrophages from dendritic cells
• MerTK is often expressed on maligt cells and may be implicated in immune evasion
• The DS5MMER antibody will work on paraformaldehyde-fixed cells
• Applications Reported: This DS5MMER antibody has been reported for use in flow cytometric analysis
• TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system
• The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response
• TCR recognition of self-peptides has been linked to autoimmune disease
• Mutant self-peptides have been associated with tumors
• Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome
• Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE)
• The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease
• Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP)
• However, a small number of T cells lack both CD4 and CD8 (double negative, DN)
• Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders
• Gamma-delta T cells are primarily found within the epithelium
• They show less TCR diversity and recognize antigens differently than alpha-beta T cells
• Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.