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7162903

BAF180, Rat anti-Human, Clone: 370.1.8C3 , Millipore Sigma™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

BAF180

Dilution

Western Blotting Analysis: A representative lot detected BAF180 in Western Blotting applications (Gao, W., et. al. (2017). Proc Natl Acad Sci U S A. 114(5):1027-1032).

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Alias

Protein polybromo-1;hPB1;BRG1-associated factor 180;Polybromo-1D

Gene Symbols

PBRM1;BAF180;PB1

Isotype

IgG2a κ

Purification Method

Protein G purified

Test Specificity

Clone 370.1.8C3 is a rat monoclonal antibody that specifically detects human Protein Polybromo-1 (BAF180). It targets an epitope within 241 amino acids from the C-terminal half.

Clone

370.1.8C3

Applications

Western Blot

Conjugate

Unconjugated

Host Species

Rat

Research Discipline

Epigenetics & Nuclear Function

Formulation

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene ID (Entrez)

NP_060783.3

Immunogen

GST-tagged recombinant fragment corresponding to 241 amino acids from the C-terminal half of human Protein polybromo-1 (BAF180).

Primary or Secondary

Primary

Content And Storage

Stable for 1 year at 2-8°C from date of receipt.

Description

  • Anti-BAF180, clone 370.1.8C3, Cat
  • No
  • MABE1138, is a highly specific rat monoclonal antibody that detects Protein Polybromo-1 (BAF180) and has been tested for use in Western Blotting
  • Protein polybromo-1 (UniProt: Q86U86; also known as hPB1, BRG1-associated factor 180, BAF180, Polybromo-1D) is encoded by the PBRM1 (also known as BAF180, PB1) gene (Gene ID: 55193) in human
  • BAF180 is a large, chromatin-remodeling protein that contains six bromodomains, two BAH-domains, and one HMG-box
  • Bromodomains recognize and bind to acetylated lysines, the BAH (bromo-adjacent homology) domains mediate protein protein interactions, and the HMG-box domain binds to DNA
  • Nine isoforms of BAF180 have been described that are produced by alternative splicing
  • BAF180 serves as a subunit of the SWI/SNF complex and plays critical roles in the regulation of chromatin remodeling and gene transcription, and is reported to be frequently mutated in several human cancers
  • BAF180 acts asa negative regulator of cell proliferation and plays a role in suppression of tumorigenesis through its ability to regulate both baseline and signal-dependent p21 transcription by its binding of the p21 promoter
  • Mutations in PBRM1 gene are known to cause renal cell carcinoma
  • (Ref.: Lee, H., et al
  • (2016)
  • Oncotarget 7(15);19134-46; Gao, W., et al
  • (2017)
  • Proc
  • Natl
  • Acad
  • Sci
  • USA
  • 114(5):1027-1032).