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7162915

CCR5 (CD195), Rat anti-Human, Clone: HEK/1/85a/7a, Millipore Sigma™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CCR5 (CD195)

Dilution

Immunocytochemistry Analysis: A representative lot detected CCR5 (CD195) in Immunocytochemistry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).Flow Cytometry Analysis: A representative lot detected CCR5 (CD195) in Flow Cytometry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Alias

C-C chemokine receptor type 5;C-C CKR-5;CC-CKR-5;CCR-5;CCR5;CHEMR13;HIV-1 fusion coreceptor;CD195

Gene Symbols

CCR5;CMKBR5

Isotype

IgG2b κ

Purification Method

Protein G purified

Test Specificity

Clone HEK/1/85a/7a is a rat monoclonal antibody that specifically detects human C-C-chemokine receptor 5 (CD195).

Clone

HEK/1/85a/7a

Applications

Flow Cytometry, Immunocytochemistry

Conjugate

Unconjugated

Host Species

Rat

Research Discipline

Inflammation & Immunology

Formulation

Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene ID (Entrez)

NP_000570.1

Immunogen

CHO cells transfected with full length human CCR-5 (CD195).

Primary or Secondary

Primary

Content And Storage

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Description

  • Anti-CCR5 (CD195), clone HEK/1/85a/7a, Cat
  • No
  • MABF2005, is a rat monoclonal antibody that detects C-C chemokine receptor type 5 (CCR5) and has been tested for use in Flow Cytometry and Immunocytochemistry
  • C-C chemokine receptor type 5 (UniProt: P51681; also known as C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195) is encoded by the CCR5 (also known as CMKBR5) gene (Gene ID: 1234) in human
  • CCR-5 is a multi-pass membrane protein that is expressed in memory T-cells, macrophages, and immature dendritic cells, and is upregulated by proinflammatory cytokines
  • It is also shown to be highly expressed in spleen, thymus, and on THP-1 and KG-1a cells
  • Lower expression is observed in ovary and lung
  • CCR-5 serves as a receptor for a number of inflammatory CC-chemokines and subsequently transduces a signal by increasing intracellular calcium levels
  • It also plays a role in the control of granulocytic lineage proliferation or differentiation
  • CCR-5 is acts as a co-receptor (CD4 being the primary receptor) of HIV-1 and HIV-2
  • It also couples to Gi class of G-proteins to inhibit cAMP production and stimulate calcium release
  • CCR-5 undergoes sulfation on two tyrosine residues in the N-terminal region, which increases efficiency of HIV-1 entry into cells
  • It also undergoes phosphorylation at C-terminal serine residues following binding to CC chemokines, especially APO-RANTES
  • Mutations in CCR5 gene have been linked to insulin-dependent diabetes mellitus
  • (Ref.: Blanpain, C., et al
  • (2002)
  • Receptors Channels
  • 8(1);19-31).