Home Products Antibodies Primary Antibodies 7162917
7162917

Siglec-H, Rat anti-Mouse, Clone: 551, Millipore Sigma™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Siglec-H

Dilution

Immunohistochemistry Analysis: A representative lot detected Siglec-H in Immunohistochemistry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).Flow Cytometry Analysis: A representative lot detected Siglec-H in Flow Cytometry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Mouse

Gene Alias

Sialic acid binding Ig-like lectin H;Siglech

Gene Symbols

Siglech;Siglec-H

Isotype

IgG1 κ

Purification Method

Protein G purified

Test Specificity

Clone 551 is a rat monoclonal antibody that detects murine Siglec-H. It targets an epitope with in the extracellular domain.

Clone

551

Applications

Flow Cytometry, Immunohistochemistry

Conjugate

Unconjugated

Host Species

Rat

Research Discipline

Inflammation & Immunology

Formulation

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene ID (Entrez)

NP_848821.2

Immunogen

Bone marrow-derived Type 1 interferon-producing cells.

Primary or Secondary

Primary

Content And Storage

Stable for 1 year at 2-8°C from date of receipt.

Description

  • Anti-Siglec-H, clone 551, Cat
  • No
  • MABF2045, is a rat monoclonal antibody that detects Sialic acid binding Ig-like lectin H and has been tested for use in Flow Cytometry and Immunohistochemistry
  • Siglec-H (UniProt: Q3Y597; also known as Sialic acid binding Ig-like lectin H) is encoded by the Siglech gene (Gene ID: 233274) in murine species
  • Siglecs are sialic acid-binding immunoglobulin-like lectins that are expressed mostly in the immune system and may be involved in both adhesive and signaling functions
  • Siglec-H is a type I, CD33-related siglec-like transmembrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off in the mature form
  • The mature form has to ig-like domains (aa 32-141 and 151-234) in its extracellular region
  • Unlike other CD33-related siglecs, it lacks tyrosine-based signaling motifs in its cytoplasmic tail
  • Siglec-H is shown to be expressed on plasmaocytoid dendritic cells (pDCs) and can serve as a reliable marker for these cells
  • Siglec-H is also reported to serve as an endocytic receptor expressed on murine pDCs
  • Action of Siglec-H depends on a 12 kDa DNAX-activating protein (DAP)
  • (Ref.: Zhang, J., et al
  • (2006)
  • Blood 107(9); 3600-3608; Yun, TJ., et al., (2016), Cell Metabolism 23(5); 852-866).