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7162943

Dab1, Rat anti-Mouse, Clone: 4E12, Millipore Sigma™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Dab1

Dilution

Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Mouse

Gene Alias

Disabled homolog 1

Gene Symbols

Dab1

Isotype

IgG2a κ

Purification Method

Protein G purified

Test Specificity

Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).

Clone

4E12

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate

Unconjugated

Host Species

Rat

Research Discipline

Neuroscience

Formulation

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene ID (Entrez)

NP_034144.1

Immunogen

GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.

Primary or Secondary

Primary

Content And Storage

Stable for 1 year at 2-8°C from date of receipt.

Description

  • Anti-Dab1, clone 4E12, Cat
  • No
  • MABN2276, is a highly specific rat monoclonal antibody that targets Disabled homolog 1 and has been tested in Immunohistochemistry, Immunoprecipitation, and Western Blotting
  • Disabled homolog 1 (UniProt: P97318; also known as DAB1) is encoded by the Dab1 gene (Gene ID: 13131) in murine species
  • Dab 1 is mainly expressed in the brain and serves as an adapter molecule involved in neural development where it is reported to direct the migration of cortical neurons past previously formed neurons to reach their proper layer
  • It is a cytosolic protein that is shown to be essential component of the reelin signaling pathway
  • It is shown to bind to the cytoplasmic tail of Reelin receptor, APO E receptor 2, and to LDL receptor
  • Dab1 can be phosphorylated on Tyr198, Tyr220, and Tyr232 upon reelin induction in embryonic neurons
  • It can also be phosphorylated on Ser524, but this phosphorylation is independent of reelin signaling
  • Tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is considered to be the most critical downstream event in Reelin signaling
  • Dab1 contains a phosphotyrosine interaction domain (PID) (aa 36-189), which specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins
  • Seven different isoforms of Dab1 have been identified that are generated via alternative splicing
  • Clone 4E12 binds to an epitope within amino acids 161-320
  • (Ref.: Onoue, A., et al
  • (2014)
  • Neurosci
  • Res
  • 88; 23-27).