CD294 (CRTH2) Monoclonal Antibody (BM16), PE-Cyanine5, eBioscience™, Invitrogen™

Description

• Description: This BM16 monoclonal antibody reacts with human CD294, which is also known as G protein-coupled receptor-44 (GPR-44) or chemoattractant receptor expressed on Th2 cells (CRTH2)
• CD294 is expressed on Th2 cells, some CD8+ cells, eosinophils, and basophils
• Moreover, CD294 binds prostaglandin D2, which promotes leukocyte chemotaxis during allergic responses
• CD294 has been reported to mediate prostaglandin D2-mediated suppression of apoptosis of Th2 cells by activating the PI3K pathway
• Applications Reported: This BM16 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This BM16 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product No
• 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
• 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• CRTH2 (GPR44) is a chemoattractant receptor-homologous molecule expressed on Th2 cells that is an identified receptor for the prostaglandin D2 (PGD2) and prostaglandin D receptor
• CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses
• However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3
• This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo
• CRTH2 is also a novel member of the G protein-coupled leukocyte chemoattractant receptor family, which is selectively expressed in Th2 but not Th1 lineage cells
• CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells
• Two transcripts have been isolated: a 3.5 kb transcript expressed primarily in the thalamus, frontal cortex, pons and hippocampus; and a 3.4 kb transcript expressed in fetal liver, leukocytes and thymus
• CRTH2 is selectively expressed in vivo in an activated state of Th2 cells including allergen-responsive Th2 cells, suggesting its pivotal roles in ongoing Th2-type immune reactions
• At least two different isoforms of CRTH2 are known to exist
• Single nucleotide polymorphisms in the 3' UTR of the CRTH2 gene have been associated with asthma susceptibility.