7190658
BrdU Polyclonal Antibody, Rockland™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
BrdU
Applications
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Rabbit
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary
Primary
Product Type
Antibody
Concentration
1.1 mg/mL
Classification
Polyclonal
Form
Liquid
Regulatory Status
RUO
Gene Alias
5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
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Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- 0 Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine
- BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication
- Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA
- Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.