Avidin Polyclonal Antibody, Biotin, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-Avidin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, as well as purified and partially purified Avidin [Hen Egg White]
• Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods
• This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons
• Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin
• The sequencing of the subunit indicates it consists of 128 amino acids
• Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions
• Avidin is stable over a wide range of pH values and temperatures
• Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification
• Pure avidin is a key component in certain immunohistochemistry (IHC) methods
• Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.