7190860

RFP Polyclonal Antibody, Biotin, Rockland™

Chicken Polyclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

RFP

Applications

ELISA, Immunofluorescence, Immunoprecipitation, Western Blot

Conjugate

Biotin

Host Species

Chicken

Formulation

0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2

Primary or Secondary

Primary

Product Type

Antibody

Concentration

1 mg/mL

Classification

Polyclonal

Form

Lyophilized

Regulatory Status

RUO

Gene Alias

RFP tag

Content And Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description

  • Store vial at 4°C prior to restoration
  • For extended storage aliquot contents and freeze at -20°C or below
  • Avoid cycles of freezing and thawing
  • Centrifuge product if not completely clear after standing at room temperature
  • This product is stable for several weeks at 4°C as an undiluted liquid
  • Dilute only prior to immediate use
  • RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above
  • RFP Antibody was tested by western blot and immunoelectrophoresis against anti-biotin, anti-chicken serum, and RFP
  • Red Fluorescent Protein (RPF) is isolated from sea anemone (Discosoma sp), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions
  • Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.