CD8b Monoclonal Antibody (SIDI8BEE), PE, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD8b
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
CD8; CD8 antigen 37 kDa chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8a; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; CD8B1; CD8beta; CD8BP; LEU2; Leu-2; LY3; Ly-3; Ly-C; Lymphocyte antigen 3; Lyt3; Lyt-3; MAL; OX-8 membrane antigen; p32; P37; T lymphocyte surface glycoprotein beta chain; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-3
Gene Symbols
Cd8b
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Cd8b
Clone
SIDI8BEE
Applications
Flow Cytometry
Conjugate
PE
Host Species
Mouse
Target Species
Human, Non-human Primate
Gene Accession No.
P10966
Gene ID (Entrez)
926
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The SIDI8BEE monoclonal antibody reacts with human and rhesus macaque CD8 beta
- The CD8b chain associates with the CD8a chain to form the CD8ab heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells
- The CD8 receptor complex binds to MHC class I and plays a role in T cell development and activation of mature T cells
- Long been thought to exist either as a heterodimer of the alpha and beta chain or as a homodimer of alpha chains, beta chains have now been shown to homodimerize as well
- SIDI8BEE can detect the CD8 beta chain in both the CD8ab heterodimer and the CD8bb homodimer
- SIDI8BEE is cross-reactive to human and rhesus macaque CD8b
- Other non-human primate species have not been tested
- Applications Reported: This SIDI8BEE antibody has been reported for use in flow cytometric analysis
- Applications Tested: This SIDI8BEE antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.125 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system
- The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules
- The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain
- Both alpha and beta chains share significant homology to immunoglobulin variable light chains
- This gene encodes the CD8 beta chain isoforms
- Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described
- A pseudogene, also located on chromosome 2, has been identified.