J Chain Antibody (Mc19-9), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
J Chain
Concentration
1.0 mg/mL
Applications
Immunohistochemistry (Frozen), Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Human
Isotype
IgG1
Purification Method
Protein A purified
Test Specificity
NB100-64825 reacts with human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques NB100-64825 detects J chain in IgM only in reducing conditions.
Clone
Mc19-9
Dilution
Western Blot 1:100-1:2000, ELISA 20 ug/ml, Immunohistochemistry 1:10-1:500, Immunoprecipitation 20 ug/ml, Immunohistochemistry-Frozen 1:200
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Immunogen
Human J Chain
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Related Products
Description
- J Chain Monoclonal specifically detects J Chain in Human samples
- It is validated for Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Frozen.