Cytokeratin 8 Antibody (AE3) - BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 5 publications
Manufacturer: Fischer Scientific
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Antigen
Cytokeratin 8
Concentration
1.0 mg/mL
Applications
Immunohistochemistry (Frozen), Flow Cytometry, Immunohistochemistry, Western Blot, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Regulatory Status
RUO
Gene Alias
CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
Gene Symbols
KRT8
Isotype
IgG1
Purification Method
Affinity Purified
Test Specificity
Recognizes most of the basic (Type II) keratins, HMW cytokeratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. AE3 reacts with all layers of epidermis (basal layer and above). The antibody is an excellent marker for distinguishing carcinomas from non-epithelial tumours. It has been used for studying the immunolocalisation of epidermal keratins, tissue distribution of keratins, various diseases of epidermis, oral mucosa and other epithelia and other epithelia development.
Clone
AE3
Dilution
Western Blot 1:100-1:2000, Flow Cytometry, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen
Classification
Monoclonal
Host Species
Mouse
Target Species
Mouse, Primate, Rabbit, Chicken, Bovine, Rat, Human
Gene ID (Entrez)
3856
Immunogen
Human epidermal cells
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Description
- Cytokeratin 8 Monoclonal specifically detects Cytokeratin 8 in Human, Mouse, Rat, Bovine, Chicken, Primate, Rabbit samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.