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7218289

CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD28

Concentration

1 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with no preservative; pH 7.2

Gene Alias

antigen CD28; CD28; CD28 antigen; CD28 antigen (Tp44); CD28 isoform; CD28 isoform 2; Cd28 molecule; CD28 precursor protein; CD28 protein; CD28 protein precursor; CD28RNA; cell surface protein; CHT28; contains partial extracellular domain; costimulatory molecule B7 receptor CD28; EGK_04709; MGC138290; sCD28; soluble CD28; T44; T-cell costimulatory molecule CD28; T-cell costimulatory molecule Tp44; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28 homolog; TP44

Gene Symbols

Cd28

Primary or Secondary

Primary

Content And Storage

4° C

Gene

Cd28

Clone

CD28.2

Applications

Flow Cytometry, Functional Assay

Conjugate

Functional Grade

Host Species

Mouse

Target Species

Human

Gene Accession No.

P10747

Gene ID (Entrez)

940

Isotype

IgG1 κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells
  • CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells
  • Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells
  • Applications Reported: The CD28.2 antibody has been reported for use in flow cytometric analysis
  • CD28.2 has also been reported in costimulation of T cells in in vitro functional assays
  • Applications Tested: TheCD28.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
  • This can be used at less than or equal to 1 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Storage and handling: Use in a sterile environment
  • Filtration: 0.2 μm post-manufacturing filtered
  • Purity: Greater than 90%, as determined by SDS-PAGE
  • Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
  • Aggregation: Less than 10%, as determined by HPLC
  • CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein
  • CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes
  • Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells
  • In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death
  • In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28
  • In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR
  • The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells
  • Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.