Connexin 32 Monoclonal Antibody (Connexin32 (2A)), Biotin, eBioscience™, Invitrogen™

Description

• The Connexin32 MAb recognizes human, mouse, rat, and chicken Connexin 32
• Connexins are a family of integral membrane proteins that form gap junction channels, which function to facilitate direct cell-cell communication
• Gap junctions are comprised of two hemichannels, or connexons, which are made up of six connexin proteins
• Over 20 connexin proteins have been identified and each combine to form either homomeric or heteromeric channels with different functional properties
• Connexin 32 is expressed by myelin, neurons, oligodendrocytes, and hepatocytes, and plays a role in the development of ovarian follicles
• Connexin 32 expression also plays a role in disease progression in chronic multiple sclerosis and experimental autoimmune encephalomyelitis
• Mutations in the Connexin 32 gene, GJB1, result in a form of Charcot-Marie-Tooth disease which is characterized by peripheral nerve dysfunction
• Applications Reported: This Connexin32 antibody has been reported for use in western blotting, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections
• Applications Tested: This Connexin32 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 10 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• This gene encodes a member of the gap junction protein family
• The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells
• According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta
• Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy
• Alternatively spliced transcript variants encoding the same protein have been found for this gene.