Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), eBioscience™, Invitrogen™

Description

• This KIKSI0803 MAb recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701
• STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members
• Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner
• Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity
• In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements
• In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements
• STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors
• STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6
• STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism
• Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure
• The STAT1 gene is located on chromosome 2
• STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors
• After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes
• Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile
• STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1)
• Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.