TIGIT Monoclonal Antibody (MBSA43), Alexa Fluor™ 660, eBioscience™, Invitrogen™

Description

• The MBSA43 monoclonal antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), which is also known as VSTM3 or WUCAM
• This 26 kDa protein is a member of the poliovirus receptor (PVR) family
• The expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but it is not expressed on B cells or naïve CD4+ T cells
• TIGIT may be upregulated on naïve CD4+ T cells upon activation
• TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK and T cells with antigen presenting cells, fibroblasts, and endothelial cells that express PVR and PVR-like proteins
• MBSA43 is reported to crossreact with Rhesus macaque
• Applications Reported: This MBSA43 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MBSA43 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.125 μg) per test
• A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700
• Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.