MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), Alexa Fluor™ 700, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
MHC Class I (H-2Kb)
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2-K1; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen
Gene Symbols
H2-K1
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
H2-K1
Clone
AF6-88.5.5.3
Applications
Flow Cytometry
Conjugate
Alexa Fluor 700
Host Species
Mouse
Target Species
Mouse
Gene Accession No.
P01901, P03991, P04223, P14428
Gene ID (Entrez)
14972
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen
- H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8
- Reactivity to other haplotypes (e.g
- d, f, j, k, p, q, r, s, u, and v) has not been observed
- Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This may be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
- Most instruments will require a 685 LP mirror and 710/20 band pass filter
- Please make sure that your instrument is capable of detecting this fluorochrome
- Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8
- HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.