7222841
CD39 Monoclonal Antibody (eBioA1 (A1)), Brilliant Ultra Violet™ 805, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD39
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias
2610206B08Rik; AA408691; ATPDase; Cd39; CD39 antigen; DKFZp686D194; DKFZp686I093; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; ecto-ATPase 1; ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1; NTPDase-1; SPG64
Gene Symbols
ENTPD1
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
ENTPD1
Clone
eBioA1 (A1)
Applications
Flow Cytometry
Conjugate
Brilliant Ultraviolet 805
Host Species
Mouse
Target Species
Human
Gene Accession No.
P49961
Gene ID (Entrez)
953
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- The eBioA1 monoclonal antibody reacts with human CD39 also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) or NTPDase
- CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer
- It is the most prominent ectoenzyme of the immune system
- The function of CD39 is to effectively remove toxic extracellular ATP by converting it to ADP or AMP
- CD39 is thought to work together with CD73 to hydrolyze ATP and has been well characterized on Langerhans cells
- Expression of CD39 was originally identified on activated lymphocytes
- Expression is also found on a subset of T cells, B cells and dendritic cells as well as weak staining on monocytes and granulocytes
- CD39 and CD73 have been found on regulatory T cells (Treg)
- Expression of CD39 on Treg may facilitate their entry into inflamed areas where high levels of ATP are present
- Expression of CD39 on Foxp3+CD4+ cells ranges from 25-45%
- Applications Reported: This eBioA1 (A1) antibody has been reported for use in flow cytometric analysis
- Applications Tested: This eBioA1 (A1) antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.5 μg) per test
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Brilliant Ultraviolet 805 is a tandem dye that emits at 797 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser
- CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes
- CD39 has intrinsic ecto-ATPase activity
- Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.