CD279 (PD-1) Monoclonal Antibody (J43), Brilliant Ultra Violet™ 805, eBioscience™, Invitrogen™

Description

• The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily
• PD-1 contains the ITIM and plays a key role in peripheral tolerance and autoimmune disease in mice
• PD-1 is expressed mainly on activated T and B lymphocytes
• Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC)
• Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells
• It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells
• When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells
• Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2
• Applications Reported: This J43 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This J43 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes
• This may be used at less than or equal to 0.5 μg per test
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Brilliant Ultraviolet 805 is a tandem dye that emits at 797 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser
• Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC)
• T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC
• PD-1 can bind two different but related ligands, PDL-1 and PDL-2
• Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of &quotedanger signals&quote such as LPS or other molecules associated with bacteria or other pathogens
• Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases
• Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.