7233098

dsRNA Mouse anti-Broad Species Reactivity, Clone: J5, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

dsRNA

Concentration

LYOPH

Applications

ELISA, Immunohistochemistry, Immunofluorescence, Immunoblot

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Multi-species

Gene Alias

Double-stranded RNA

Isotype

IgG2b κ

Purification Method

Protein A purified

Content And Storage

Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.

Clone

J5

Dilution

ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoblotting

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

Lyophilised from PBS

Immunogen

Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.

Primary or Secondary

Primary

Reconstitution

The lyophilised sample should be reconstituted with 200 ul (500 ul for 500 ug size) sterile distilled water. The mAb will then be in PBS without any stabilisers or preservatives at a concentration of 1 mg/ml.

Description

  • dsRNA Monoclonal antibody specifically detects dsRNA in Broad Species Reactivity samples
  • It is validated for ELISA, Immunohistochemistry, Immunofluorescence, Immunoblot