CD45R (B220) Monoclonal Antibody (RA3-6B2), Brilliant Ultra Violet™ 661, eBioscience™, Invitrogen™

Description

• The RA3-6B2 mAb reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule
• CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells
• However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen
• Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.25μg per test Brilliant Ultra Violet 661 (BUV661) is a tandem dye that emits at 660 nm and is intended for use on cytometers equipped with an Ultraviolet (355 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Excitation: 350 nm; Emission: 660 nm; Laser: Ultraviolet Laser
• CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family
• PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation
• CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains
• CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling
• CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
• CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains
• Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
• In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
• CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
• Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.