75-798-9782

Vimentin Monoclonal Antibody (V9), Alexa Fluor™ Plus 750, Invitrogen™

Manufacturer: LIFE TECHNOLOGIES

Select a Size

Pack Size SKU Availability Price
Each of 1 75-798-9782-Each-of-1 In Stock ₹ 44,055.00

75-798-9782 - Each of 1

₹ 44,055.00

In Stock

Quantity

1

Base Price: ₹ 44,055.00

GST (18%): ₹ 7,929.90

Total Price: ₹ 51,984.90

Antigen

Vimentin

Classification

Monoclonal

Concentration

0.2 mg/mL

Formulation

PBS with 0.5% BSA, 10% proprietary stabilizer and 0.05% sodium azide; pH 7.2

Gene Accession No.

P08670

Host Species

Mouse

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

Applications

Immunohistochemistry (Paraffin)

Clone

V9

Conjugate

Alexa Fluor Plus 750

Gene

Vim

Gene Alias

cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

7431

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

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Description

  • Vimentin Monoclonal antibody specifically detects Vimentin in Human samples
  • It is validated for Immunohistochemistry (Paraffin) Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments
  • Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells
  • It is proposed to be a marker of cardiac differentiation
  • In neural cells, vimentin expression is gradually replaced by neurofilaments
  • Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils
  • This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin
  • Applications Reported: This V9 antibody has been reported for use in western blotting, immunocytochemistry, and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P)
  • Applications Tested: This V9 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 1.33 μg/mL
  • It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest
  • Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment
  • This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.